DAP3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DAP3 full-length
Host
Human
Theoretical MW (kDa)
45.6
Interspecies Antigen Sequence
Mouse (81); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DAP3 antibody (H00007818-B02) by Western Blots.
SDS-PAGE Gel
DAP3 transfected lysate
Western Blot
Lane 1: DAP3 transfected lysate ( 45.6 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DAP3
Entrez GeneID
7818GeneBank Accession#
NM_004632Protein Accession#
NP_004623Gene Name
DAP3
Gene Alias
DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10
Gene Description
death associated protein 3
Omim ID
602074Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq
Other Designations
OTTHUMP00000033480|OTTHUMP00000033481|death-associated protein 3|mitochondrial 28S ribosomal protein S29
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Interactome
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