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DAP3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007818-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DAP3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 45.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DAP3 antibody (H00007818-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007818-T02
    DAP3 transfected lysate
    Western Blot
    QC Testing of H00007818-T02
    Lane 1: DAP3 transfected lysate ( 45.6 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7818
  • Gene Name:
  • DAP3
  • Gene Alias:
  • DAP-3,DKFZp686G12159,MGC126058,MGC126059,MRP-S29,MRPS29,bMRP-10
  • Gene Description:
  • death associated protein 3
  • Gene Summary:
  • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000033480,OTTHUMP00000033481,death-associated protein 3,mitochondrial 28S ribosomal protein S29
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