ZYX monoclonal antibody (M01), clone 2C10-4A7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.
Lane 1: ZYX transfected lysate(61.3 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # H00007791-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ZYX on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (88.66 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ZYX.
Immunogen
ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYECEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (85)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (88.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.
Lane 1: ZYX transfected lysate(61.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # H00007791-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to ZYX on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ZYX
Entrez GeneID
7791GeneBank Accession#
BC008743Protein Accession#
AAH08743Gene Name
ZYX
Gene Alias
ESP-2, HED-2
Gene Description
zyxin
Omim ID
602002Gene Ontology
HyperlinkGene Summary
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq
Other Designations
-
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Early committed polarization of intracellular tension in response to cell shape determines the osteogenic differentiation of mesenchymal stromal cells.
Ming-Chung Wu, Helen Wenshin Yu, Yin-Quan Chen, Meng-Hsin Ou, Ricardo Serrano, Guan-Lin Huang, Yang-Kao Wang, Kung-Hui Lin, Yu-Jui Fan, Chi-Chang Wu, Juan C Del Álamo, Arthur Chiou, Shu Chien, Jean-Cheng Kuo.
Acta Biomaterialia 2022 Nov; S1742-7061(22):709.
Application：IF, Human, Human mesenchymal stromal cells.
-
Defective VWF secretion due to expression of MYH9-RD E1841K mutant in endothelial cells disrupts hemostasis.
Yang Cao, Yanjie Sun, Yanan Deng, Guoqin Wei, Junling Liu, Shengyu Jin, Chao Dong, Xuya Kang, Yingqing Huo, Jingjing Zhang, Jincai Luo.
Blood Advances 2022 Aug; 6(15):4537.
Application：WB-Ce, Human, HUVECs cells.
-
Structural Colored Balloon Composed of Temperature-Responsive Polymers Showing LCST Behavior.
Higashiguchi K, Morita N, Matsuda K.
Langmuir 2018 Sep; [Epub].
Application：IF, WB, Human, HeLa cells.
-
Tensile loads on tethered actin filaments induce accumulation of cell adhesion-associated proteins in vitro.
Kiyoshima D, Tatsumi H, Hirata H, Sokabe M.
Langmuir 2018 Sep; [Epub].
Application：IF, WB, Human, HeLa cells.
-
NBR1 enables autophagy-dependent focal adhesion turnover.
Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, Wittmann T, Debnath J.
The Journal of Cell Biology 2016 Feb; 212(5):577.
Application：WB, Human, MCF 10A-Ras cells.
-
Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.
Hirata H, Tatsumi H, Sokabe M.
Journal of Cell Science 2008 Aug; 121(Pt 17):2795.
Application：IF, Human, Human foreskin fibroblasts.
-
Early committed polarization of intracellular tension in response to cell shape determines the osteogenic differentiation of mesenchymal stromal cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com