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ZP2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007783-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ZP2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 81.3
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ZP2 antibody (H00007783-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007783-T01
    ZP2 transfected lysate.
    Western Blot
    QC Testing of H00007783-T01
    Lane 1: ZP2 transfected lysate ( 81.3 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7783
  • Gene Name:
  • ZP2
  • Gene Alias:
  • ZPA
  • Gene Description:
  • zona pellucida glycoprotein 2 (sperm receptor)
  • Gene Summary:
  • The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. [provided by RefSeq
  • Other Designations:
  • zona pellucida glycoprotein 2,zona pellucida protein A,zona pellucida sperm-binding protein 2
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