TRIM25 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TRIM25 transfected lysate.
Western Blot
Lane 1: TRIM25 transfected lysate ( 71.00 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRIM25 full-length
Host
Human
Theoretical MW (kDa)
71
Interspecies Antigen Sequence
Mouse (73); Rat (71)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRIM25 antibody (H00007706-D01P) by Western Blots.
SDS-PAGE Gel
TRIM25 transfected lysate.
Western Blot
Lane 1: TRIM25 transfected lysate ( 71.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRIM25
Entrez GeneID
7706GeneBank Accession#
BC016924.1Protein Accession#
AAH16924.1Gene Name
TRIM25
Gene Alias
EFP, RNF147, Z147, ZNF147
Gene Description
tripartite motif-containing 25
Omim ID
600453Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq
Other Designations
estrogen-responsive finger protein|tripartite motif protein TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
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Interactome
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Disease
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