SF1 monoclonal antibody (M01), clone 2E12

Catalog # H00007536-M01

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Size:100 ug
Price: USD $ 335.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

SF1 monoclonal antibody (M01), clone 2E12. Western Blot analysis of SF1 expression in HeLa.

Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

SF1 monoclonal antibody (M01), clone 2E12. Western Blot analysis of SF1 expression in NIH/3T3.

Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.

Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).
Lane 2: Non-transfected lysate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Immunoprecipitation
Application

Immunoprecipitation

Immunoprecipitation of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SF1 monoclonal antibody.

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a capture antibody.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescence of monoclonal antibody to SF1 on HeLa cell . [antibody concentration 10 ug/ml]

QC Test

Western Blot detection against Immunogen (37.84 KDa) .

  • Specification

    Product Description

    Mouse monoclonal antibody raised against a partial recombinant SF1.

    Immunogen

    SF1 (NP_004621, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERH

    Host

    Mouse

    Reactivity

    Human, Mouse

    Isotype

    IgG2a Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (37.84 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Cell lysate)

    SF1 monoclonal antibody (M01), clone 2E12. Western Blot analysis of SF1 expression in HeLa.

    Western Blot (Cell lysate)

    SF1 monoclonal antibody (M01), clone 2E12. Western Blot analysis of SF1 expression in NIH/3T3.

    Western Blot (Transfected lysate)

    Western Blot analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.

    Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).
    Lane 2: Non-transfected lysate.

    Western Blot (Recombinant protein)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunoperoxidase of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

    Immunoprecipitation

    Immunoprecipitation of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SF1 monoclonal antibody.

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a capture antibody.

    ELISA

    Immunofluorescence

    Immunofluorescence of monoclonal antibody to SF1 on HeLa cell . [antibody concentration 10 ug/ml]
  • Gene Info — SF1

    Entrez GeneID

    7536

    GeneBank Accession#

    NM_004630

    Protein Accession#

    NP_004621

    Gene Name

    SF1

    Gene Alias

    D11S636, ZFM1, ZNF162

    Gene Description

    splicing factor 1

    Omim ID

    601516

    Gene Ontology

    Hyperlink

    Other Designations

    OTTHUMP00000069749|OTTHUMP00000069750|OTTHUMP00000069752|zinc finger protein 162

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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