YWHAZ 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
YWHAZ transfected lysate
Western Blot
Lane 1: YWHAZ transfected lysate ( 27.7 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-YWHAZ full-length
Host
Human
Theoretical MW (kDa)
27.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-YWHAZ antibody (H00007534-B01) by Western Blots.
SDS-PAGE Gel
YWHAZ transfected lysate
Western Blot
Lane 1: YWHAZ transfected lysate ( 27.7 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — YWHAZ
Entrez GeneID
7534GeneBank Accession#
NM_003406Protein Accession#
NP_003397Gene Name
YWHAZ
Gene Alias
KCIP-1, MGC111427, MGC126532, MGC138156
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Omim ID
601288Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq
Other Designations
14-3-3 protein/cytosolic phospholipase A2|14-3-3 zeta|OTTHUMP00000165851|OTTHUMP00000165852|OTTHUMP00000165854|OTTHUMP00000165858|OTTHUMP00000165859|OTTHUMP00000165860|phospholipase A2|protein kinase C inhibitor protein-1|tyrosine 3/tryptophan 5 -monooxyg
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Interactome
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Pathway
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Disease
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