XBP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
XBP1 transfected lysate.
Western Blot
Lane 1: XBP1 transfected lysate ( 28.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-XBP1 full-length
Host
Human
Theoretical MW (kDa)
28.7
Interspecies Antigen Sequence
Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-XBP1 antibody (H00007494-D01) by Western Blots.
SDS-PAGE Gel
XBP1 transfected lysate.
Western Blot
Lane 1: XBP1 transfected lysate ( 28.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — XBP1
Entrez GeneID
7494GeneBank Accession#
NM_005080.2Protein Accession#
NP_005071.2Gene Name
XBP1
Gene Alias
TREB5, XBP2
Gene Description
X-box binding protein 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq
Other Designations
OTTHUMP00000028682|X-box-binding protein-1
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Interactome
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