WT1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WT1 full-length
Host
Human
Theoretical MW (kDa)
34.5
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WT1 antibody (H00007490-D01P) by Western Blots.
SDS-PAGE Gel
WT1 transfected lysate.
Western Blot
Lane 1: WT1 transfected lysate ( 34.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WT1
Entrez GeneID
7490GeneBank Accession#
BC032861.2Protein Accession#
AAH32861.1Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
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Interactome
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Disease
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