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WT1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007490-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-WT1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 34.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-WT1 antibody (H00007490-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007490-T02
    WT1 transfected lysate.
    Western Blot
    QC Testing of H00007490-T02
    Lane 1: WT1 transfected lysate ( 34.50 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7490
  • Gene Name:
  • WT1
  • Gene Alias:
  • Gene Description:
  • Wilms tumor 1
  • Gene Summary:
  • This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
  • Other Designations:
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