WHSC2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WHSC2 transfected lysate.
Western Blot
Lane 1: WHSC2 transfected lysate ( 57.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WHSC2 full-length
Host
Human
Theoretical MW (kDa)
57.3
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WHSC2 antibody (H00007469-B03P) by Western Blots.
SDS-PAGE Gel
WHSC2 transfected lysate.
Western Blot
Lane 1: WHSC2 transfected lysate ( 57.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WHSC2
Entrez GeneID
7469GeneBank Accession#
NM_005663.2Protein Accession#
NP_005654.2Gene Name
WHSC2
Gene Alias
FLJ10442, FLJ25112, NELF-A, NELFA, P/OKcl.15
Gene Description
Wolf-Hirschhorn syndrome candidate 2
Omim ID
606026Gene Ontology
HyperlinkGene Summary
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq
Other Designations
Wolf-Hirschhorn syndrome candidate 2 protein
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Interactome
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Disease
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