WEE1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WEE1 full-length
Host
Human
Theoretical MW (kDa)
71.7
Interspecies Antigen Sequence
Mouse (90); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WEE1 antibody (H00007465-D01P) by Western Blots.
SDS-PAGE Gel
WEE1 transfected lysate.
Western Blot
Lane 1: WEE1 transfected lysate ( 71.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WEE1
Entrez GeneID
7465GeneBank Accession#
BC051831.1Protein Accession#
AAH51831.1Gene Name
WEE1
Gene Alias
DKFZp686I18166, FLJ16446, WEE1A, WEE1hu
Gene Description
WEE1 homolog (S. pombe)
Omim ID
193525Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq
Other Designations
WEE1+ homolog|wee1 tyrosine kinase|wee1-like protein kinase
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Interactome
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Pathway
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Disease
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