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Last updated: 2016/12/4
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VRK2 monoclonal antibody (M01), clone 3B10

  • Catalog # : H00007444-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant VRK2.
  • Immunogen:
  • VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • VQTAKTNLLDELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKAHNRLI
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00007444-M01
    Western Blot detection against Immunogen (36.74 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • VRK2 monoclonal antibody (M01), clone 3B10. Western Blot analysis of VRK2 expression in U-2 OS ( Cat # L022V1 ).
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • VRK2 monoclonal antibody (M01), clone 3B10 Western Blot analysis of VRK2 expression in K-562 ( Cat # L009V1 ).
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  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody (M01), clone 3B10.

    Lane 1: VRK2 transfected lysate(58.1 KDa).
    Lane 2: Non-transfected lysate.
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  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged VRK2 is approximately 1ng/ml as a capture antibody.
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  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi ( Cat # H00007444-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody (M01) clone 3B10 (Cat # H00007444-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
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  • Gene Information
  • Entrez GeneID:
  • 7444
  • Gene Name:
  • VRK2
  • Gene Alias:
  • -
  • Gene Description:
  • vaccinia related kinase 2
  • Gene Summary:
  • This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • vaccinia virus B1R-related kinase 2,vaccinia-related kinase-2
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