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Last updated: 2016/11/27

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VRK1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007443-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-VRK1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 45.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-VRK1 antibody (H00007443-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007443-T02
    VRK1 transfected lysate.
    Western Blot
    QC Testing of H00007443-T02
    Lane 1: VRK1 transfected lysate ( 45.50 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7443
  • Gene Name:
  • VRK1
  • Gene Alias:
  • MGC117401,MGC138280,MGC142070
  • Gene Description:
  • vaccinia related kinase 1
  • Gene Summary:
  • This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq
  • Other Designations:
  • vaccinia virus B1R-related kinase 1,vaccinia-related kinase-1
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