VIM (Human) IP-WB Antibody Pair
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More Files
- More Functions
Immunoprecipitation of VIM transfected lysate using mouse monoclonal anti-VIM and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-VIM.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of VIM transfected lysate using mouse monoclonal anti-VIM and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-VIM.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: mouse monoclonal anti-VIM (300 ug)
2. Antibody pair for WB: rabbit polyclonal anti-VIM (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — VIM
Entrez GeneID
7431Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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