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Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 CX Grade

  • Catalog # : H00007431-M08J
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody recognizes human cell-surface vimentin (CSV).
  • Immunogen:
  • Human recombinant vimentin
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Cell-Surface Vimentin (CSV) detecting antibody is best used before cell fixation and permeabilization. If fixation is required, please use Abnova’s Special Fixative.

    Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation.
  • Interspecies Antigen Sequence:
  • Mouse (100); Rat (100)
  • Applications
  • Immunofluorescence
  • Immunofluorescence enlargeenlarge this image
  • Immunofluorescence staining on non-fixed, non-permeabilized MDA-MB-231 cells using FITC conjugated Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 for CSV (Green) and DAPI for nucleus (Blue).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of CSV on isolated LM7 cancer cells and leukocytes using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. The cells were co-stained against DRAQ5, CSV and CD45. (Satelli A., et al. Cancer Research 2014; 74:1645-1650)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged VIM is 0.1 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of CSV expression in cancer (top) and normal (bottom) cell lines using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Isotype controls were used as negative controls. (Satelli A., et al., Clinical Cancer Research 2014; 21(4):899-906)
  • Application Image
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7431
  • Gene Name:
  • VIM
  • Gene Alias:
  • FLJ36605
  • Gene Description:
  • vimentin
  • Gene Summary:
  • This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
  • Other Designations:
  • OTTHUMP00000019224
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