Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1
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More Files
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Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Western Blot analysis of VIM expression in PC-3.
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Western Blot analysis of VIM expression in MDA-MB-231.
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Western Blot analysis of VIM expression in SKNBE2.
Immunofluorescence
Immunofluorescence staining on non-fixed, non-permeabilized MDA-MB-231 cells using FITC conjugated Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 for CSV (Green) and DAPI for nucleus (Blue).
Immunofluorescence
Immunofluorescence staining of CSV on isolated LM7 cancer cells and leukocytes using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. The cells were co-stained against DRAQ5, CSV and CD45. (Satelli A., et al. Cancer Research 2014; 74:1645-1650)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VIM is 0.1 ng/ml as a capture antibody.
Flow Cytometry
Flow cytometric analysis of CSV expression in cancer (top) and normal (bottom) cell lines using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Isotype controls were used as negative controls. (Satelli A., et al., Clinical Cancer Research 2014; 21(4):899-906)
Western Blot detection against Immunogen.
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Specification
Product Description
Mouse monoclonal antibody recognizes human cell-surface vimentin (CSV).
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
Human recombinant vimentin
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Form
Liquid
Preparation Method
Cell Culture Production
Isotype
IgG2b, kappa
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
Cell-Surface Vimentin (CSV) detecting antibody is best used before cell fixation and permeabilization. If fixation is required, please use Abnova’s Special Fixative.
Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation. -
Applications
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Western Blot analysis of VIM expression in PC-3.Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Western Blot analysis of VIM expression in MDA-MB-231.Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Western Blot analysis of VIM expression in SKNBE2.Western Blot (Recombinant protein)
Immunofluorescence
Immunofluorescence staining on non-fixed, non-permeabilized MDA-MB-231 cells using FITC conjugated Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 for CSV (Green) and DAPI for nucleus (Blue).Immunofluorescence
Immunofluorescence staining of CSV on isolated LM7 cancer cells and leukocytes using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. The cells were co-stained against DRAQ5, CSV and CD45. (Satelli A., et al. Cancer Research 2014; 74:1645-1650)Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VIM is 0.1 ng/ml as a capture antibody.ELISA
Flow Cytometry
Flow cytometric analysis of CSV expression in cancer (top) and normal (bottom) cell lines using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Isotype controls were used as negative controls. (Satelli A., et al., Clinical Cancer Research 2014; 21(4):899-906) -
Gene Info — VIM
Entrez GeneID
7431Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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Publication Reference
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A surrogate marker for very early-stage tau pathology is detectable by molecular magnetic resonance imaging.
Parag Parekh, Qingshan Mu, Andrew Badachhape, Rohan Bhavane, Mayank Srivastava, Laxman Devkota, Xianwei Sun, Prajwal Bhandari, Jason L Eriksen, Eric Tanifum, Ketan Ghaghada, Ananth Annapragada.
Theranostics 2022 Jul; 12(12):5504.
Application：IF, Mouse, Brain tissue, H-SY5Y cell.
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Glioblastoma Multiforme Selective Nanomedicines for Improved Anti-Cancer Treatments.
Jason Thomas Duskey, Arianna Rinaldi, Ilaria Ottonelli, Riccardo Caraffi, Chiara Alessia De Benedictis, Ann Katrin Sauer, Giovanni Tosi, Maria Angela Vandelli, Barbara Ruozi, Andreas Martin Grabrucker.
Pharmaceutics 2022 Jul; 14(7):1450.
Application：Immunogen, Rat, C6 cells.
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A surrogate marker for very early-stage tau pathology is detectable by molecular magnetic resonance imaging.
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