VIM (Human) Recombinant Protein
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More Files
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SDS-PAGE Gel
Western Blot
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Specification
Product Description
Purified VIM (NP_003371.2 , 408 a.a. - 466 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293T cells
Sequence
ESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
Host
Human
Theoretical MW (kDa)
31.24
Interspecies Antigen Sequence
Mouse (97); Rat (98)
Form
Liquid
Preparation Method
Transfection of pSuper-VIM plasmid into HEK293T cell, and the expressed protein was purified by protein A.
Purification
Protein A purification
Concentration
>= 10 ug/ml
Quality Control Testing
SDS-PAGE and Western Blot
SDS-PAGE Gel
Western Blot
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — VIM
Entrez GeneID
7431GeneBank Accession#
NM_003380.2Protein Accession#
NP_003371.2Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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