VIL1 monoclonal antibody (M26), clone 2F10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of VIL1 expression in transfected 293T cell line by VIL1 monoclonal antibody (M26), clone 2F10.
Lane 1: VIL1 transfected lysate(46.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VIL1 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (71.83 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant VIL1.
Immunogen
VIL1 (AAH17303, 1 a.a. ~ 421 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVGEGQAGAVREPGSRSWARRATWSTTHPPSLTCIFNEDFYAGSGLVLADGDVDKL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (71.83 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of VIL1 expression in transfected 293T cell line by VIL1 monoclonal antibody (M26), clone 2F10.
Lane 1: VIL1 transfected lysate(46.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VIL1 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — VIL1
Entrez GeneID
7429GeneBank Accession#
BC017303Protein Accession#
AAH17303Gene Name
VIL1
Gene Alias
D2S1471, VIL
Gene Description
villin 1
Omim ID
193040Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq
Other Designations
OTTHUMP00000164145
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