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Last updated: 2016/10/16
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VEGF 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007422-T01
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-VEGF full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 17.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-VEGF antibody (H00007422-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007422-T01
    VEGF transfected lysate
    Western Blot
    QC Testing of H00007422-T01
    Lane 1: VEGF transfected lysate ( 17.2 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7422
  • Protein Accession#:
  • -
  • Gene Name:
  • Gene Alias:
  • Gene Description:
  • vascular endothelial growth factor A
  • Gene Summary:
  • This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
  • Other Designations:
  • vascular endothelial growth factor isoform VEGF165,vascular permeability factor
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