VASP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: VASP transfected lysate ( 39.8 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
VASP transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VASP full-length
Host
Human
Theoretical MW (kDa)
41.91
Interspecies Antigen Sequence
Mouse (84); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VASP antibody (H00007408-B01) by Western Blots.
Western Blot
Lane 1: VASP transfected lysate ( 39.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
VASP transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VASP
Entrez GeneID
7408GeneBank Accession#
NM_003370Protein Accession#
NP_003361Gene Name
VASP
Gene Alias
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Gene Description
vasodilator-stimulated phosphoprotein
Omim ID
601703Gene Ontology
HyperlinkGene Summary
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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