USF1 monoclonal antibody (M02), clone 2A7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant USF1.
Immunogen
USF1 (NP_009053, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGT
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
USF1 monoclonal antibody (M02), clone 2A7 Western Blot analysis of USF1 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged USF1 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — USF1
Entrez GeneID
7391GeneBank Accession#
NM_007122Protein Accession#
NP_009053Gene Name
USF1
Gene Alias
FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, bHLHb11
Gene Description
upstream transcription factor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000029691|OTTHUMP00000029693|major late transcription factor 1|upstream stimulatory factor 1
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Interactome
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Disease
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Publication Reference
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Association of thymidylate synthase enhancer region polymorphisms with thymidylate synthase activity in vivo.
de Bock CE, Garg MB, Scott N, Sakoff JA, Scorgie FE, Ackland SP, Lincz LF.
The Pharmacogenomics Journal 2011 Aug; 11(4):307.
Application:WB-Ce, Human, HCT-116, HeLa, WiDr cells .
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Use of a single chain antibody library for ovarian cancer biomarker discovery.
Ramirez AB, Loch CM, Zhang Y, Liu Y, Wang X, Wayner EA, Sargent JE, Sibani S, Hainsworth E, Mendoza EA, Eugene R, Labaer J, Urban ND, McIntosh MW, Lampe PD.
Molecular and Cellular Proteomics 2010 May; 9(7):1449.
Application:Dot, Human, Recombinant protein, Transfected HEK 293T cells.
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A role of DNA-PK for the metabolic gene regulation in response to insulin.
Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS.
Cell 2009 Mar; 136(6):1056.
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Association of thymidylate synthase enhancer region polymorphisms with thymidylate synthase activity in vivo.
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