USF1 monoclonal antibody (M01), clone 3F6
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant USF1.
Immunogen
USF1 (AAH35505, 1 a.a. ~ 310 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (59.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
USF1 monoclonal antibody (M01), clone 3F6 Western Blot analysis of USF1 expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of USF1 expression in transfected 293T cell line by USF1 monoclonal antibody (M01), clone 3F6.
Lane 1: USF1 transfected lysate(33.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged USF1 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of USF1 over-expressed 293 cell line, cotransfected with USF1 Validated Chimera RNAi ( Cat # H00007391-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with USF1 monoclonal antibody (M01), clone 3F6 (Cat # H00007391-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — USF1
Entrez GeneID
7391GeneBank Accession#
BC035505Protein Accession#
AAH35505Gene Name
USF1
Gene Alias
FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, bHLHb11
Gene Description
upstream transcription factor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000029691|OTTHUMP00000029693|major late transcription factor 1|upstream stimulatory factor 1
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Interactomes
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Diseases
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Publication Reference
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A role of DNA-PK for the metabolic gene regulation in response to insulin.
Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS.
Cell 2009 Mar; 136(6):1056.
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A role of DNA-PK for the metabolic gene regulation in response to insulin.
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