UNG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: UNG transfected lysate ( 33.9 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
UNG transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UNG full-length
Host
Human
Theoretical MW (kDa)
33.55
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UNG antibody (H00007374-B01) by Western Blots.
Western Blot
Lane 1: UNG transfected lysate ( 33.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
UNG transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UNG
Entrez GeneID
7374GeneBank Accession#
NM_003362Protein Accession#
NP_003353Gene Name
UNG
Gene Alias
DGU, DKFZp781L1143, HIGM4, UDG, UNG1, UNG15, UNG2
Gene Description
uracil-DNA glycosylase
Gene Ontology
HyperlinkGene Summary
This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. [provided by RefSeq
Other Designations
uracil-DNA glycosylase 1, uracil-DNA glycosylase 2|uracil-DNA glycosylase 2
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Interactome
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Pathway
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Disease
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