UCHL1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
UCHL1 transfected lysate.
Western Blot
Lane 1: UCHL1 transfected lysate ( 24.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UCHL1 full-length
Host
Human
Theoretical MW (kDa)
24.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UCHL1 antibody (H00007345-D01P) by Western Blots.
SDS-PAGE Gel
UCHL1 transfected lysate.
Western Blot
Lane 1: UCHL1 transfected lysate ( 24.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UCHL1
Entrez GeneID
7345GeneBank Accession#
NM_004181.3Protein Accession#
NP_004172.2Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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Interactome
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Disease
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