UCHL1 monoclonal antibody (M01), clone 1B8-4D2
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More Files
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Western Blot (Cell lysate)
UCHL1 monoclonal antibody (M01), clone 1B8-4D2 Western Blot analysis of UCHL1 expression in IMR-32 ( Cat # L008V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of UCHL1 expression in transfected 293T cell line by UCHL1 monoclonal antibody (M01), clone 1B8-4D2.
Lane 1: UCHL1 transfected lysate (Predicted MW: 24.8 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to UCHL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ~ 10 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged UCHL1 is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (50.27 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant UCHL1.
Immunogen
UCHL1 (AAH00332, 1 a.a. ~ 223 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (50.27 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
UCHL1 monoclonal antibody (M01), clone 1B8-4D2 Western Blot analysis of UCHL1 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Transfected lysate)
Western Blot analysis of UCHL1 expression in transfected 293T cell line by UCHL1 monoclonal antibody (M01), clone 1B8-4D2.
Lane 1: UCHL1 transfected lysate (Predicted MW: 24.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to UCHL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ~ 10 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged UCHL1 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — UCHL1
Entrez GeneID
7345GeneBank Accession#
BC000332Protein Accession#
AAH00332Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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Interactome
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Disease
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