UBTF monoclonal antibody (M04), clone 2D8
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More Files
- More Functions
Western Blot (Cell lysate)
UBTF monoclonal antibody (M04), clone 2D8. Western Blot analysis of UBTF expression in NIH/3T3(Cat # L018V1 ).
Western Blot (Cell lysate)
UBTF monoclonal antibody (M04), clone 2D8. Western Blot analysis of UBTF expression in PC-12(Cat # L012V1 ).
Western Blot (Cell lysate)
UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2 ( Cat # L019V1 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant UBTF.
Immunogen
UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYIS
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
UBTF monoclonal antibody (M04), clone 2D8. Western Blot analysis of UBTF expression in NIH/3T3(Cat # L018V1 ).Western Blot (Cell lysate)
UBTF monoclonal antibody (M04), clone 2D8. Western Blot analysis of UBTF expression in PC-12(Cat # L012V1 ).Western Blot (Cell lysate)
UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2 ( Cat # L019V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml] -
Gene Info — UBTF
Entrez GeneID
7343GeneBank Accession#
NM_014233Protein Accession#
NP_055048Gene Name
UBTF
Gene Alias
NOR-90, UBF
Gene Description
upstream binding transcription factor, RNA polymerase I
Omim ID
600673Gene Ontology
HyperlinkGene Summary
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM
Other Designations
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