UBE2N monoclonal antibody (M02), clone 3G1-D10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant UBE2N.
Immunogen
UBE2N (AAH03365, 1 a.a. ~ 152 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (99)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (42.46 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
UBE2N monoclonal antibody (M02), clone 3G1-D10 Western Blot analysis of UBE2N expression in LNCaP ( Cat # L004V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged UBE2N is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to UBE2N on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — UBE2N
Entrez GeneID
7334GeneBank Accession#
BC003365Protein Accession#
AAH03365Gene Name
UBE2N
Gene Alias
MGC131857, MGC8489, UBC13, UbcH-ben
Gene Description
ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Omim ID
603679Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq
Other Designations
bendless-like ubiquitin conjugating enzyme|ubiquitin carrier protein N|ubiquitin-conjugating enzyme E2N|ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)|ubiquitin-protein ligase N|yeast UBC13 homolog
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Interactome
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Pathway
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Disease
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