UBE2B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: UBE2B transfected lysate ( 16.72 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
UBE2B transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UBE2B full-length
Host
Human
Theoretical MW (kDa)
16.83
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UBE2B antibody (H00007320-B01) by Western Blots.
Western Blot
Lane 1: UBE2B transfected lysate ( 16.72 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
UBE2B transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UBE2B
Entrez GeneID
7320GeneBank Accession#
BC008470Protein Accession#
AAH08470Gene Name
UBE2B
Gene Alias
E2-17kDa, HHR6B, HR6B, RAD6B, UBC2
Gene Description
ubiquitin-conjugating enzyme E2B (RAD6 homolog)
Omim ID
179095Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq
Other Designations
E2 protein|ubiquitin carrier protein B|ubiquitin-conjugating enzyme E2B|ubiquitin-protein ligase B
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Interactome
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Pathway
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Disease
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