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UBE2B 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007320-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-UBE2B full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 16.83
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-UBE2B antibody (H00007320-B01) by Western Blots.
    Western Blot
    QC Testing of H00007320-T01
    Lane 1: UBE2B transfected lysate ( 16.72 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00007320-T01
    UBE2B transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7320
  • Gene Name:
  • UBE2B
  • Gene Alias:
  • E2-17kDa,HHR6B,HR6B,RAD6B,UBC2
  • Gene Description:
  • ubiquitin-conjugating enzyme E2B (RAD6 homolog)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq
  • Other Designations:
  • E2 protein,ubiquitin carrier protein B,ubiquitin-conjugating enzyme E2B,ubiquitin-protein ligase B
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