UBE1L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
UBA7 transfected lysate.
Western Blot
Lane 1: UBA7 transfected lysate ( 111.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UBE1L full-length
Host
Human
Theoretical MW (kDa)
111.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UBE1L antibody (H00007318-D01P) by Western Blots.
SDS-PAGE Gel
UBA7 transfected lysate.
Western Blot
Lane 1: UBA7 transfected lysate ( 111.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UBA7
Entrez GeneID
7318GeneBank Accession#
NM_003335.2Protein Accession#
NP_003326.2Gene Name
UBA7
Gene Alias
D8, MGC12713, UBA1B, UBE1L, UBE2
Gene Description
ubiquitin-like modifier activating enzyme 7
Omim ID
191325Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq
Other Designations
UBA1, ubiquitin-activating enzyme E1 homolog B|UBA7, ubiquitin-activating enzyme E1|ubiquitin-activating enzyme E1-like|ubiquitin-activating enzyme E1-related protein|ubiquitin-activating enzyme-2
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Interactome
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Disease
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