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TYMS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007298-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-TYMS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 35.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-TYMS antibody (H00007298-D01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007298-T02
    TYMS transfected lysate.
    Western Blot
    QC Testing of H00007298-T02
    Lane 1: TYMS transfected lysate ( 35.70 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7298
  • Gene Name:
  • TYMS
  • Gene Alias:
  • HsT422,MGC88736,TMS,TS,TSase
  • Gene Description:
  • thymidylate synthetase
  • Gene Summary:
  • Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq
  • Other Designations:
  • Thymidylate synthase
  • Related Disease
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