TNFSF4 (Human) Recombinant Protein
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Specification
Product Description
Purified TNFSF4 (NP_003317.1, 52 a.a. - 183 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293T cells
Sequence
VSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL
Host
Human
Theoretical MW (kDa)
19.8
Interspecies Antigen Sequence
Rat (46)
Form
Liquid
Preparation Method
Transfection of pSuper-TNFSF4 plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Quality Control Testing
SDS-PAGE and Western Blot
SDS-PAGE Gel
Western Blot
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — TNFSF4
Entrez GeneID
7292GeneBank Accession#
NM_003326.2Protein Accession#
NP_003317.1Gene Name
TNFSF4
Gene Alias
CD134L, CD252, GP34, OX-40L, OX4OL, TXGP1
Gene Description
tumor necrosis factor (ligand) superfamily, member 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF4/OX4. It is found to be involved in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells. [provided by RefSeq
Other Designations
CD134 ligand|OTTHUMP00000032704|OX40 antigen ligand|OX40 ligand|glycoprotein 34 kd|tax-transcriptionally activated glycoprotein 1 (34kD)
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Pathway
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Disease
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