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Last updated: 2016/11/27

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HIRA 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007290-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HIRA full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 111.8
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HIRA antibody (H00007290-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007290-T01
    HIRA transfected lysate.
    Western Blot
    QC Testing of H00007290-T01
    Lane 1: HIRA transfected lysate ( 111.8 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7290
  • Protein Accession#:
  • -
  • Gene Name:
  • HIRA
  • Gene Alias:
  • DGCR1,TUP1,TUPLE1
  • Gene Description:
  • HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq
  • Other Designations:
  • DiGeorge critical region gene 1,HIR histone cell cycle regulation defective homolog A
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