Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi ( Cat # H00007277-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody (M01), clone 2E11 (Cat # H00007277-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq