TUBA1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human TUBA1 full-length ORF ( AAH09238, 1 a.a. - 448 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
75.02
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — TUBA4A
Entrez GeneID
7277GeneBank Accession#
BC009238Protein Accession#
AAH09238Gene Name
TUBA4A
Gene Alias
FLJ30169, H2-ALPHA, TUBA1
Gene Description
tubulin, alpha 4a
Omim ID
191110Gene Ontology
HyperlinkGene Summary
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq
Other Designations
tubulin alpha-1 chain|tubulin, alpha 1 (testis specific)
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of ?-tubulin as an autoantigen recognized by sera from patients with neuropsychiatric systemic lupus erythematosus.
Ndhlovu M, PreuB BE, Dengjel J, Stevanovic S, Weiner SM, Klein R.
Brain, Behavior, and Immunity 2011 Feb; 25(2):279.
Application：SDS-PAGE, Recombinant proteins.
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Identification of ?-tubulin as an autoantigen recognized by sera from patients with neuropsychiatric systemic lupus erythematosus.
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