TTK monoclonal antibody (M01), clone 3G7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TTK.
Immunogen
TTK (AAH00633, 1 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVP
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (44.99 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TTK monoclonal antibody (M01), clone 3G7 Western Blot analysis of TTK expression in K-562 ( Cat # L009V1 ).Western Blot (Cell lysate)
TTK monoclonal antibody (M01), clone 3G7. Western Blot analysis of TTK expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TTK expression in transfected 293T cell line by TTK monoclonal antibody (M01), clone 3G7.
Lane 1: TTK transfected lysate(97.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TTK is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — TTK
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Interactome
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Pathway
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Disease
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Publication Reference
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High frequency of TTK mutations in microsatellite-unstable colorectal cancer and evaluation of their effect on spindle-assembly checkpoint.
Niittymaki I, Gylfe A, Laine L, Laakso M, Lehtonen HJ, Kondelin J, Tolvanen J, Nousiainen K, Pouwels J, Jarvinen H, Nuorva K, Mecklin JP, Makinen M, Ristimaki A, Orntoft TF, Hautaniemi S, Karhu A, Kallio MJ, Aaltonen LA.
Carcinogenesis 2011 Mar; 32(3):305.
Application:WB, Human, LoVo, HCT8, HCT116, HCA7, VACO5, RKO cells.
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High frequency of TTK mutations in microsatellite-unstable colorectal cancer and evaluation of their effect on spindle-assembly checkpoint.
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