Characterization of human thymic exosomes. Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall OPLoS One. 2013 Jul 2;8(7):e67554. doi: 10.1371/journal.pone.0067554. Print 2013.
Western blot analysis of TSG101 over-expressed 293 cell line, cotransfected with TSG101 Validated Chimera RNAi ( Cat # H00007251-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TSG101 monoclonal antibody (M01), clone 5B7 (Cat # H00007251-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq