Western blot analysis of NR2C2 over-expressed 293 cell line, cotransfected with NR2C2 Validated Chimera RNAi ( Cat # H00007182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR2C2 monoclonal antibody (M01), clone 2A5 (Cat # H00007182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).[supplied by OMIM