TPO 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TPO full-length
Host
Human
Theoretical MW (kDa)
102.74
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TPO antibody (H00007173-B01) by Western Blots.
Western Blot
Lane 1: TPO transfected lysate ( 102.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
TPO transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TPO
Entrez GeneID
7173GeneBank Accession#
BC095448Protein Accession#
AAH95448Gene Name
TPO
Gene Alias
MSA, TPX
Gene Description
thyroid peroxidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000115304|OTTHUMP00000115536|thyroid microsomal antigen|thyroperoxidase
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Pathway
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Disease
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