TPMT monoclonal antibody (M01), clone 1B5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TPMT.
Immunogen
TPMT (AAH05339, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (79); Rat (67)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.69 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TPMT monoclonal antibody (M01), clone 1B5 Western Blot analysis of TPMT expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M01), clone 1B5.
Lane 1: TPMT transfected lysate(28.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TPMT on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1 ug/ml]ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TPMT on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — TPMT
Entrez GeneID
7172GeneBank Accession#
BC005339Protein Accession#
AAH05339Gene Name
TPMT
Gene Alias
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Gene Description
thiopurine S-methyltransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q. [provided by RefSeq
Other Designations
OTTHUMP00000016076|S-adenosyl-L-methionine:thiopurine S-methyltransferase|thiopurine methyltransferase
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Interactome
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Pathway
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Disease
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Publication Reference
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S-adenosylmethionine regulates thiopurine methyltransferase activity and decreases 6-mercaptopurine cytotoxicity in MOLT lymphoblasts.
Milek M, Karas Kuzelicki N, Smid A, Mlinaric-Rascan I.
Biochemical Pharmacology 2009 Jun; 77(12):1845.
Application:Flow Cyt, Human, MOLT cells.
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A novel gene delivery system for stable transfection of thiopurine-S-methyltransferase gene in versatile cell types.
Egle R, Milek M, Mlinaric-Rascan I, Fahr A, Kristl J.
European Journal of Pharmaceutics and Biopharmaceutics 2007 Oct; 69(1):23.
Application:WB, Human, HepG2, HEK 293, Jurkat cells.
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S-adenosylmethionine regulates thiopurine methyltransferase activity and decreases 6-mercaptopurine cytotoxicity in MOLT lymphoblasts.
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