TNXB monoclonal antibody (M01), clone 2D3-1B10
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of TNXB expression in transfected 293T cell line by TNXB monoclonal antibody (M01), clone 2D3-1B10.
Lane 1: TNXB transfected lysate (Predicted MW: 74 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of TNXB transfected lysate using anti-TNXB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TNXB MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNXB is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (99.77 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TNXB.
Immunogen
TNXB (AAH33740, 1 a.a. ~ 673 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MRLSWSVAQGPFDSFVVQYEDTNGQPQALLVDGDQSKILISGLEPSTPYRFLLYGLHEGKRLGPLSAEGTTGLAPAGQTSEESRPRLSQLSVTDVTTSSLRLNWEAPPGAFDSFLLRFGVPSPSTLEPHPRPLLQRELMVPGTRHSAVLRDLRSGTLYSLTLYGLRGPHKADSIQGTARTLSPVLESPRDLQFSEIRETSAKVNWMPPPSRADSFKVSYLLADGGEPQSVQVDGQARTQKLQGLIPGARYEVTVVSVRGFEESEPLTGFLTTVPDGPTQLRALNLTEGFAVLHWKPPQNPVDTYDVQVTAPGAPPLQAETPGSAVDYPLHDLVLHTNYTATVRGLRGPNLTSPASITFTTGLEAPRDLEAKEVTPRTALLTWTEPPVRPAGYLLSFHTPGGQNQEILLPGGITSHQLLGLFPSTSYNARLQAMWGQSLLPPVSTSFTTGGLRIPFPRDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNISGEFWLGNEALHSLTQAGDYSMRVDLRAGDEAVFAQYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNSLLISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFRSPAGGG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (99.77 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TNXB expression in transfected 293T cell line by TNXB monoclonal antibody (M01), clone 2D3-1B10.
Lane 1: TNXB transfected lysate (Predicted MW: 74 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of TNXB transfected lysate using anti-TNXB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TNXB MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNXB is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — TNXB
Entrez GeneID
7148GeneBank Accession#
BC033740Protein Accession#
AAH33740Gene Name
TNXB
Gene Alias
HXBL, TENX, TNX, TNXB1, TNXB2, TNXBS, XB, XBS
Gene Description
tenascin XB
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000062664|growth-inhibiting protein 45|hexabrachion-like|tenascin XB1|tenascin XB2|tenascin-X
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Interactome
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Pathway
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Disease
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