TNNT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: TNNT1 transfected lysate ( 27.61 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
TNNT1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TNNT1 full-length
Host
Human
Theoretical MW (kDa)
9.13
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TNNT1 antibody (H00007138-B01) by Western Blots.
Western Blot
Lane 1: TNNT1 transfected lysate ( 27.61 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
TNNT1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TNNT1
Entrez GeneID
7138GeneBank Accession#
NM_003283Protein Accession#
NP_003274Gene Name
TNNT1
Gene Alias
ANM, FLJ98147, MGC104241, STNT, TNT, TNTS
Gene Description
troponin T type 1 (skeletal, slow)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
slow skeletal muscle troponin T|troponin T1, skeletal, slow|troponin-T1, skeletal, slow
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Interactome
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