Western blot analysis of TNNI3 over-expressed 293 cell line, cotransfected with TNNI3 Validated Chimera RNAi ( Cat # H00007137-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TNNI3 monoclonal antibody (M04), clone 1E7 (Cat # H00007137-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq
familial hypertrophic cardiomyopathy 7,troponin I, cardiac