TNNI2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TNNI2 transfected lysate.
Western Blot
Lane 1: TNNI2 transfected lysate ( 21.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TNNI2 full-length
Host
Human
Theoretical MW (kDa)
21.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TNNI2 antibody (H00007136-D01P) by Western Blots.
SDS-PAGE Gel
TNNI2 transfected lysate.
Western Blot
Lane 1: TNNI2 transfected lysate ( 21.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TNNI2
Entrez GeneID
7136GeneBank Accession#
NM_003282Protein Accession#
NP_003273.1Gene Name
TNNI2
Gene Alias
AMCD2B, DA2B, FSSV
Gene Description
troponin I type 2 (skeletal, fast)
Gene Ontology
HyperlinkGene Summary
This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000014141|OTTHUMP00000014142|troponin I fast twitch 2|troponin I, fast-twitch skeletal muscle isoform|troponin I, skeletal, fast
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