TIMP2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TIMP2 full-length
Host
Human
Theoretical MW (kDa)
24.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TIMP2 antibody (H00007077-B01) by Western Blots.
SDS-PAGE Gel
TIMP2 transfected lysate.
Western Blot
Lane 1: TIMP2 transfected lysate ( 24.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TIMP2
Entrez GeneID
7077GeneBank Accession#
NM_003255Protein Accession#
NP_003246Gene Name
TIMP2
Gene Alias
CSC-21K
Gene Description
TIMP metallopeptidase inhibitor 2
Omim ID
188825Gene Ontology
HyperlinkGene Summary
This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq
Other Designations
tissue inhibitor of metalloproteinase 2|tissue inhibitor of metalloproteinases 2
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