TIA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TIA1 transfected lysate
Western Blot
Lane 1: TIA1 transfected lysate ( 24.1 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TIA1 full-length
Host
Human
Theoretical MW (kDa)
24.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TIA1 antibody (H00007072-B01) by Western Blots.
SDS-PAGE Gel
TIA1 transfected lysate
Western Blot
Lane 1: TIA1 transfected lysate ( 24.1 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TIA1
Entrez GeneID
7072GeneBank Accession#
BC015944Protein Accession#
AAH15944Gene Name
TIA1
Gene Alias
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Gene Description
TIA1 cytotoxic granule-associated RNA binding protein
Omim ID
603518Gene Ontology
HyperlinkGene Summary
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq
Other Designations
T-cell-restricted intracellular antigen-1|TIA1 cytotoxic granule-associated RNA-binding protein|TIA1 protein|cytotoxic granule-associated RNA-binding protein|nucleolysin TIA-1 isoform p40|p40-TIA-1 (containing p15-TIA-1)
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