TGM2 monoclonal antibody (M10), clone 2F4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TGM2.
Immunogen
TGM2 (AAH03551, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLE
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.83 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TGM2 expression in transfected 293T cell line by TGM2 monoclonal antibody (M10), clone 2F4.
Lane 1: TGM2 transfected lysate(61.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TGM2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TGM2 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TGM2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — TGM2
Entrez GeneID
7052GeneBank Accession#
BC003551Protein Accession#
AAH03551Gene Name
TGM2
Gene Alias
G-ALPHA-h, GNAH, TG2, TGC
Gene Description
transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Omim ID
190196Gene Ontology
HyperlinkGene Summary
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
C polypeptide|OTTHUMP00000030960|TGase C|TGase-H|protein-glutamine-gamma-glutamyltransferase|tissue transglutaminase|transglutaminase 2|transglutaminase C
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Interactome
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Disease
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Publication Reference
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Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach.
Xue H, Lu B, Zhang J, Wu M, Huang Q, Wu Q, Sheng H, Wu D, Hu J, Lai M.
Journal of Proteome Research 2010 Jan; 9(1):545.
Application:WB, Human, SW480, SW620 cells.
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Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach.
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