TGM1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: TGM1 transfected lysate ( 89.87 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
TGM1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TGM1 full-length
Host
Human
Theoretical MW (kDa)
89.98
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TGM1 antibody (H00007051-B02) by Western Blots.
Western Blot
Lane 1: TGM1 transfected lysate ( 89.87 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
TGM1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TGM1
Entrez GeneID
7051GeneBank Accession#
BC034699Protein Accession#
AAH34699Gene Name
TGM1
Gene Alias
ICR2, KTG, LI, LI1, TGASE, TGK
Gene Description
transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq
Other Designations
TGase K|epidermal TGase|transglutaminase 1|transglutaminase K|transglutaminase, keratinocyte
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Interactome
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Disease
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