TGFB1I1 monoclonal antibody (M01), clone 4B2-D8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TGFB1I1.
Immunogen
TGFB1I1 (AAH32545, 1 a.a. ~ 444 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERAGKPYCQPCFLKLFG
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.58 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 Western Blot analysis of TGFB1I1 expression in Hela ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 monoclonal antibody (M01), clone 4B2-D8.
Lane 1: TGFB1I1 transfected lysate(47.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TGFB1I1 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TGFB1I1 over-expressed 293 cell line, cotransfected with TGFB1I1 Validated Chimera RNAi ( Cat # H00007041-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 (Cat # H00007041-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — TGFB1I1
Entrez GeneID
7041GeneBank Accession#
BC032545Protein Accession#
AAH32545Gene Name
TGFB1I1
Gene Alias
ARA55, HIC-5, HIC5, TSC-5
Gene Description
transforming growth factor beta 1 induced transcript 1
Omim ID
602353Gene Ontology
HyperlinkGene Summary
This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
androgen receptor coactivator ARA55
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Interactome
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