TFPI 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TFPI full-length
Host
Human
Theoretical MW (kDa)
35
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TFPI antibody (H00007035-D01P) by Western Blots.
SDS-PAGE Gel
TFPI transfected lysate.
Western Blot
Lane 1: TFPI transfected lysate ( 35.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TFPI
Entrez GeneID
7035GeneBank Accession#
NM_006287.4Protein Accession#
NP_006278.1Gene Name
TFPI
Gene Alias
EPI, LACI, TFI, TFPI1
Gene Description
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Omim ID
152310Gene Ontology
HyperlinkGene Summary
This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq
Other Designations
OTTHUMP00000163472|anti-convertin|extrinsic pathway inhibitor|lipoprotein-associated coagulation inhibitor|tissue factor pathway inhibitor
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Interactome
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Pathway
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Disease
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