Western blot analysis of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi ( Cat # H00007023-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody (M03), clone 7A10 (Cat # H00007023-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM
transcription factor AP-4 (activating enhancer-binding protein 4)