TFAP4 monoclonal antibody (M03), clone 7A10
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More Files
- More Functions
Western Blot (Cell lysate)
TFAP4 monoclonal antibody (M03), clone 7A10 Western Blot analysis of TFAP4 expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Transfected lysate)
Western Blot analysis of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody (M03), clone 7A10.
Lane 1: TFAP4 transfected lysate(38.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi ( Cat # H00007023-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody (M03), clone 7A10 (Cat # H00007023-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TFAP4.
Immunogen
TFAP4 (NP_003214, 93 a.a. ~ 192 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TFAP4 monoclonal antibody (M03), clone 7A10 Western Blot analysis of TFAP4 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody (M03), clone 7A10.
Lane 1: TFAP4 transfected lysate(38.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi ( Cat # H00007023-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody (M03), clone 7A10 (Cat # H00007023-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — TFAP4
Entrez GeneID
7023GeneBank Accession#
NM_003223Protein Accession#
NP_003214Gene Name
TFAP4
Gene Alias
AP-4, bHLHc41
Gene Description
transcription factor AP-4 (activating enhancer binding protein 4)
Omim ID
600743Gene Ontology
HyperlinkGene Summary
Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM
Other Designations
transcription factor AP-4 (activating enhancer-binding protein 4)
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Interactome
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Disease
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