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Last updated: 2017/10/22

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TEF 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007008-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-TEF full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 33.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-TEF antibody (H00007008-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007008-T01
    TEF transfected lysate.
    Western Blot
    QC Testing of H00007008-T01
    Lane 1: TEF transfected lysate ( 33.2 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7008
  • Protein Accession#:
  • -
  • Gene Name:
  • TEF
  • Gene Alias:
  • -
  • Gene Description:
  • thyrotrophic embryonic factor
  • Gene Summary:
  • Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • thyrotroph embryonic factor
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